Genetic Analysis of Dengue Virus in Severe and Non-Severe Cases in Dhaka, Bangladesh, in 2018-2022.

Dengue virus (DENV) infections have unpredictable clinical outcomes, ranging from asymptomatic or minor febrile illness to severe and fatal disease. The severity of dengue infection is at least partly related to the replacement of circulating DENV serotypes and/or genotypes. To describe clinical profiles of patients and the viral sequence diversity corresponding to non-severe and severe cases, we collected patient samples from 2018 to 2022 at Evercare Hospital Dhaka, Bangladesh. Serotyping of 495 cases and sequencing of 179 cases showed that the dominant serotype of DENV shifted from DENV2 in 2017 and 2018 to DENV3 in 2019. DENV3 persisted as the only representative serotype until 2022. Co-circulation of clades B and C of the DENV2 cosmopolitan genotype in 2017 was replaced by circulation of clade C alone in 2018 with all clones disappearing thereafter. DENV3 genotype I was first detected in 2017 and was the only genotype in circulation until 2022. We observed a high incidence of severe cases in 2019 when the DENV3 genotype I became the only virus in circulation. Phylogenetic analysis revealed clusters of severe cases in several different subclades of DENV3 genotype I. Thus, these serotype and genotype changes in DENV may explain the large dengue outbreaks and increased severity of the disease in 2019.

Library Services during COVID-19 Pandemic: A Bibliometric Analysis and knowledge Mapping

The increased usage of libraries during the Covid-19 pandemic has been well known to science communicators, but scientific communication has not evaluated the distributions of its published findings. Thus, this study intends to identify and map library services in international publishing, particularly the Scopus database during the pandemic. Sixty-four publications in the Scopus database were assessed using the bibliometric approach and fifty-seven documents are considered. To find documents from January 2020-June 2021, the author utilized keywords such as library services AND covid-19. Microsoft excels and VOSviewer software was used to analyze the result and visualize the knowledge map. The results showed that most of the papers are dealt with favorable and adoptive library services techniques, implementation of different users demands during the pandemic. The study found the most prolific authors. Rafiq, M., Batool, S. H., Ali, A. F., & Ullah, M. received a maximum of 20 citations from a single document and Michalak R., Ortiz-Díaz E.M., Pacheco-Mendoza J., Rysavy M.D.T., Saavedra-Alamillas C. published 2 documents each. The United States of America is ranked top to publish the scholarly publication on the Library Services during the COVID-19 related documents with 21 documents. India is in the 2nd rank with eight papers, followed by Pakistan, China, Nigeria, Peru, and South Korea. As a result, publications concerning this topic have a high likelihood of emerging. The most frequent terms used by authors are COVID-19(22.90%), library services (7.01%), academic libraries (5.61%). The shift towards advanced virtual and digital services offers more than just boosting librarians and library patrons;it serves as a move to keep the library relevant in a tech-centered society.